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Cellosaurus ICGi052-B-1 (CVCL_E9B9)

[Text version]
Cell line name ICGi052-B-1
Synonyms PD57-7 MT8
Accession CVCL_E9B9
Resource Identification Initiative To cite this cell line use: ICGi052-B-1 (RRID:CVCL_E9B9)
Comments From: The Federal Research Center Institute of Cytology and Genetics; Novosibirsk; Russia.
Population: Caucasian.
Characteristics: Upon doxycycline induction expresses a MitoTimer biosensor. MitoTimer is a DsRed-Timer protein fused to the transit peptide of COX18 so as to target it to the mitochondrial matrix (PubMed=40149527; DOI=10.3390/biomedicines13030550; PMCID=PMC11940372).
Characteristics: Using CRISPR/Cas9 a Neo-M2rtTA construct and a Puro-TRE-mCMV-MitoTimer were introduced at the AAVS1 safe harbor locus (PubMed=40149527).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 55DQE; DsRed-Timer.
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations
  • Mutation; HGNC; HGNC:6893; MAPT; Simple; p.Asn279Lys (c.837T>G) (N596K, c.1788T>G or N614K, c.1842T>G); ClinVar=VCV000014253; Zygosity=Heterozygous (PubMed=40149527).
Disease Frontotemporal dementia-1 (NCIt: C217087)
Behavioral variant of frontotemporal dementia (ORDO: Orphanet_275864)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E9B8 (ICGi052-B)
Sex of cell Female
Age at sampling 46Y
Category Induced pluripotent stem cell
Publications

PubMed=40149527; DOI=10.3390/biomedicines13030550; PMCID=PMC11940372
Julia A. Nadtochy, Sergey P. Medvedev, Elena V. Grigor'eva, Sofia V. Pavlova, Julia M. Minina, Anton V. Chechushkov, Anastasia A. Malakhova, Liudmila V. Kovalenko, Suren Minasovich Zakian;
Transgenic iPSC lines with genetically encoded MitoTimer to study mitochondrial biogenesis in dopaminergic neurons with tauopathy.
Biomedicines 13:550.1-550.18(2025)

Cross-references
Cell line databases/resources hPSCreg; ICGi052-B-1
Biological sample resources BioSamples; SAMEA117400231
Entry history
Entry creation14-Aug-2025
Last entry update14-Aug-2025
Version number1