Cellosaurus cell line Jurkat-NFAT-Luc2-CD3D-KO-1B1 (CVCL

Cellosaurus Jurkat-NFAT-Luc2-CD3D-KO-1B1 (CVCL_E8IY)

[Text version]

Cell line name

Jurkat-NFAT-Luc2-CD3D-KO-1B1

Accession

CVCL_E8IY

Resource Identification Initiative

To cite this cell line use: Jurkat-NFAT-Luc2-CD3D-KO-1B1 (RRID:CVCL_E8IY)

Comments

Population: Caucasian.
Characteristics: The luciferase reporter gene is under the control of the control of NFAT response element (from parent cell line).
Doubling time: ~26 hours (KYinno=KC-4083).
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:1673; CD3D.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase (Note=With optimized codon usage for mammalian expression = Luc2).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.

Sequence variations

Mutation; HGNC; HGNC:959; BAX; Simple; p.Glu41Argfs*19 (c.121delG); ClinVar=VCV000009512; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:959; BAX; Simple; p.Glu41Glyfs*33 (c.121dupG); ClinVar=VCV000009511; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:6079; INPP5D; Simple; p.Gln345Ter (c.1033C>T); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:6079; INPP5D; Simple; c.1097+1065_1097+1112del47; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:7325; MSH2; Simple; p.Arg711Ter (c.2131C>T); ClinVar=VCV000090903; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; HGNC:7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg196Ter (c.586C>T); ClinVar=VCV000043589; Zygosity=Heterozygous (from parent cell line).

Disease

Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_E8IE (Jurkat-NFAT-Luc2 reporter)
Children:

CVCL_E8IP (Jurkat-NFAT-Luc2-CD3D-CD3E-KO-1C4)

CVCL_E8IQ (Jurkat-NFAT-Luc2-CD3D-CD3E-KO-2A3)

Sex of cell

Male

Age at sampling

14Y