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Cellosaurus HS2_L1HS-CRISPRi_g1 (CVCL_E8I7)

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Cell line name HS2_L1HS-CRISPRi_g1
Accession CVCL_E8I7
Resource Identification Initiative To cite this cell line use: HS2_L1HS-CRISPRi_g1 (RRID:CVCL_E8I7)
Comments Population: Japanese.
Characteristics: Transduced with the FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP vector that contains dCas9 fused to a KRAB repressive domain, a LINE-1 targeting gRNA (gL1HSg1) and eGFP (DOI=10.1101/2025.01.17.633315).
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_DQ30 (648A1)
Sex of cell Male
Age at sampling 30-39Y
Category Induced pluripotent stem cell
Publications

DOI=10.1101/2025.01.17.633315
Anita Adami, Raquel Garza, Patricia Gerdes, Pia Annette Johansson, Fereshteh Dorazehi, Symela Koutounidou, Laura Castilla-Vallmanya, Diahann Alexandra Maria Atacho, Yogita Sharma, Jenny G. Johansson, Oliver H. Tam ...Show all 16 authors... , Agnete Kirkeby, Roger Alistair Barker, Molly C. Gale-Hammell, Christopher H. Douse, Johan Jakobsson; Show fewer authors
LINE-1 retrotransposons regulate the exit of human pluripotency and early brain development.
bioRxiv 2025:01.17.633315-01.17.633315(2025)

Cross-references
Gene expression databases GEO; GSM8667521
GEO; GSM8667522
GEO; GSM8667523
Entry history
Entry creation14-Aug-2025
Last entry update14-Aug-2025
Version number1