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Cellosaurus HS1_L1HS-CRISPRi_LacZ (CVCL_E8I6)

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Cell line name HS1_L1HS-CRISPRi_LacZ
Accession CVCL_E8I6
Resource Identification Initiative To cite this cell line use: HS1_L1HS-CRISPRi_LacZ (RRID:CVCL_E8I6)
Comments Characteristics: Control cell line for HS1_L1HS-CRISPRi_g1 (Cellosaurus=CVCL_E8I4) and HS1_L1HS-CRISPRi_g2 (Cellosaurus=CVCL_E8I5).
Characteristics: Transduced with the FUGW U6 gLacZ dCas9-KRAB-T2a-GFP vector that contains dCas9 fused to a KRAB repressive domain, a lacZ targeting gRNA and eGFP (DOI=10.1101/2025.01.17.633315).
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
Omics: Proteomics.
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_DQ11 (606A1)
Sex of cell Female
Age at sampling <1D
Category Induced pluripotent stem cell
Publications

DOI=10.1101/2025.01.17.633315
Anita Adami, Raquel Garza, Patricia Gerdes, Pia Annette Johansson, Fereshteh Dorazehi, Symela Koutounidou, Laura Castilla-Vallmanya, Diahann Alexandra Maria Atacho, Yogita Sharma, Jenny G. Johansson, Oliver H. Tam ...Show all 16 authors... , Agnete Kirkeby, Roger Alistair Barker, Molly C. Gale-Hammell, Christopher H. Douse, Johan Jakobsson; Show fewer authors
LINE-1 retrotransposons regulate the exit of human pluripotency and early brain development.
bioRxiv 2025:01.17.633315-01.17.633315(2025)

Cross-references
Gene expression databases GEO; GSM8667515
GEO; GSM8667516
GEO; GSM8667517
GEO; GSM8667530
GEO; GSM8667531
GEO; GSM8667532
Proteomic databases PRIDE; PXD059661
Entry history
Entry creation14-Aug-2025
Last entry update14-Aug-2025
Version number1