Cellosaurus HEK293 ATG2A/ATG2B DKO GFP-ATG2A TagBFP-LC3B (CVCL_E8GV)
Cell line name | HEK293 ATG2A/ATG2B DKO GFP-ATG2A TagBFP-LC3B |
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Synonyms | HEK293 ATG2 DKO + GFP-ATG2A + TagBFP-LC3B |
Accession | CVCL_E8GV |
Resource Identification Initiative | To cite this cell line use: HEK293 ATG2A/ATG2B DKO GFP-ATG2A TagBFP-LC3B (RRID:CVCL_E8GV) |
Comments | Characteristics: Transduced with a pLVX-Neo-TagBFP-LC3B construct (DOI=10.1101/2025.03.24.645038). Characteristics: Transduced with a eGFP-ATG2A construct (from parent cell line). Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:29028; ATG2A. Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:20187; ATG2B. Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=FPbase; BFJKS; TagBFP (Note=Blue fluorescent protein derived from eqFP578). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:29028; ATG2A. Genetic integration: Method=Transduction; Gene=HGNC; HGNC:13352; MAP1LC3B. Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo. Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_E8CH (HEK293 ATG2A/2B DKO GFP-ATG2A) |
Sex of cell | Female |
Age at sampling | Fetus |
Category | Transformed cell line |
Publications | DOI=10.1101/2025.03.24.645038 |
Entry history | |
Entry creation | 14-Aug-2025 |
Last entry update | 14-Aug-2025 |
Version number | 1 |