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Cellosaurus HEK293 ATG2A/ATG2B DKO GFP-ATG2A TagBFP-LC3B (CVCL_E8GV)

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Cell line name HEK293 ATG2A/ATG2B DKO GFP-ATG2A TagBFP-LC3B
Synonyms HEK293 ATG2 DKO + GFP-ATG2A + TagBFP-LC3B
Accession CVCL_E8GV
Resource Identification Initiative To cite this cell line use: HEK293 ATG2A/ATG2B DKO GFP-ATG2A TagBFP-LC3B (RRID:CVCL_E8GV)
Comments Characteristics: Transduced with a pLVX-Neo-TagBFP-LC3B construct (DOI=10.1101/2025.03.24.645038).
Characteristics: Transduced with a eGFP-ATG2A construct (from parent cell line).
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:29028; ATG2A.
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:20187; ATG2B.
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=FPbase; BFJKS; TagBFP (Note=Blue fluorescent protein derived from eqFP578).
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:29028; ATG2A.
Genetic integration: Method=Transduction; Gene=HGNC; HGNC:13352; MAP1LC3B.
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E8CH (HEK293 ATG2A/2B DKO GFP-ATG2A)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

DOI=10.1101/2025.03.24.645038
Devin M. Fuller, Yu-Mei Wu, Florian Schueder, Burha Rasool, Shanta Nag, Justin L. Korfhage, Rolando Garcia-Milian, Katerina D. Melnyk, Joerg Bewersdorf, Pietro De Camilli, Thomas J. Melia;
ATG2A engages Rab1a and ARFGAP1 positive membranes during autophagosome biogenesis.
bioRxiv 2025:03.24.645038-03.24.645038(2025)

Entry history
Entry creation14-Aug-2025
Last entry update14-Aug-2025
Version number1