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Cellosaurus S2-LiveDropGFP-M4 (CVCL_E8G3)

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Cell line name S2-LiveDropGFP-M4
Accession CVCL_E8G3
Resource Identification Initiative To cite this cell line use: S2-LiveDropGFP-M4 (RRID:CVCL_E8G3)
Comments Group: Insect cell line.
Characteristics: Transfected with LiveDrop, a sensitive probe used to identify the earliest steps of lipid droplet formation. LiveDrop contains the membrane hairpin domain (AA 160-216) of Gpat4 fused to eGFP (PubMed=35274690).
Genetic integration: Method=Transfection; Gene=FlyBase_Gene; FBgn0034971; Gpat4 (Note=With p.Met1_Arg159del and p.Leu217_Thr458del = 160-216).
Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276.
Hierarchy Parent: CVCL_Z232 (Schneider 2)
Sex of cell Male
Age at sampling Late embryonic stage
Category Spontaneously immortalized cell line
Publications

PubMed=35274690; DOI=10.1242/jcs.259092
Kazuki Ueda, Matthew N. Anderson-Baron, Julie Haskins, Sarah C. Hughes, Andrew James Simmonds;
Recruitment of peroxin 14 to lipid droplets affects lipid storage in Drosophila.
J. Cell Sci. 135:jcs259092.1-jcs259092.18(2022)

Cross-references
Cell line collections (Providers) DGRC; 352
Cell line databases/resources FlyBase_Cell_line; FBtc0000352
Entry history
Entry creation14-Aug-2025
Last entry update14-Aug-2025
Version number1