Cellosaurus S2R+-MT-sfGFP-TurboID (CVCL_E8FZ)
| Cell line name | S2R+-MT-sfGFP-TurboID |
|---|---|
| Accession | CVCL_E8FZ |
| Resource Identification Initiative | To cite this cell line use: S2R+-MT-sfGFP-TurboID (RRID:CVCL_E8FZ) |
| Comments | Group: Insect cell line. Characteristics: Transfected with a MT-sfGFP-TurboID construct containing a fusion of sfGFP with TurboID, a modified biotin ligase used for proximity labeling to identify proteins interacting with a protein of interest. The expression of the fusion protein is driven by a copper-inducible metallothionein promoter (DGRC=350). Genetic integration: Method=Transfection; Gene=FPbase; B4SOW; Superfolder GFP (Note=sfGFP, superfolding variant of GFP). Genetic integration: Method=Transfection; Gene=UniProtKB; P06709; Escherichia coli birA (Note=With p.Gln65Pro, p.Ile87Val, p.Arg118Ser, p.Glu140Lys, p.Gln141Arg, p.Ala146del, p.Ser150Gly, p.Leu151Pro, p.Val160Ala, p.Thr192Ala, p.Lys194Ile, p.Met209Val, p.Met241Thr, p.Ser263Pro and p.Ile305Val = TurboID). Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922. |
| Species of origin | Drosophila melanogaster (Fruit fly)
(NCBI Taxonomy: 7227)
Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276. |
| Hierarchy | Parent: CVCL_Z831 (S2R+) |
| Sex of cell | Male |
| Age at sampling | Late embryonic stage |
| Category | Spontaneously immortalized cell line |
| Cross-references | |
| Cell line collections (Providers) | DGRC; 350 |
| Cell line databases/resources | FlyBase_Cell_line; FBtc0000350 |
| Entry history | |
| Entry creation | 14-Aug-2025 |
| Last entry update | 14-Aug-2025 |
| Version number | 1 |