Cellosaurus HEK293T ASNS-Flag-Y398Lfs*4 (CVCL_E8E2)
| Cell line name | HEK293T ASNS-Flag-Y398Lfs*4 |
|---|---|
| Accession | CVCL_E8E2 |
| Resource Identification Initiative | To cite this cell line use: HEK293T ASNS-Flag-Y398Lfs*4 (RRID:CVCL_E8E2) |
| Comments | Characteristics: Transduced with a ASNS(Y398Lfs*4)-Flag-PuroR-IRES-eGFP construct (PubMed=36873094). Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=HGNC; HGNC:753; ASNS (Note=With p.Tyr398Leufs*4 (c.1192dupT) and a C-terminal Flag tag). Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo. Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen. Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA). Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0063 (HEK293T) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Publications | PubMed=36873094; DOI=10.1002/jmd2.12356; PMCID=PMC9981421 |
| Entry history | |
| Entry creation | 10-Apr-2025 |
| Last entry update | 14-Aug-2025 |
| Version number | 2 |