Cellosaurus Applied StemCell DR4-MEF irradiated (CVCL_E8D2)
| Cell line name | Applied StemCell DR4-MEF irradiated |
|---|---|
| Accession | CVCL_E8D2 |
| Resource Identification Initiative | To cite this cell line use: Applied StemCell DR4-MEF irradiated (RRID:CVCL_E8D2) |
| Comments | Characteristics: Expresses transgenes that make this cell line resistant to three different drugs: geneticin (G418), puromycin and hygromycin. KO of Hprt1 makes the cell line resistant to 6-tioguanine (PubMed=9278500). Characteristics: Used as feeder cells to support the growth of stem cells in the undifferentiated state. Characteristics: Growth-arrested by irradiation. Knockout cell: Method=KO mouse; MGI; MGI:96217; Hprt1. Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P13249; S.alboniger pac (PuroR). Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCIt; C44386; Gamma radiation. Derived from site: In situ; Embryo; UBERON=UBERON_0000922. Cell type: Fibroblast; CL=CL_0000057. |
| Species of origin | Mus musculus (Mouse)
(NCBI Taxonomy: 10090)
Breed/subspecies: DR4. |
| Sex of cell | Sex unspecified |
| Age at sampling | 13-14FD |
| Category | Finite cell line |
| Web pages | Provider; ASC; ASF-1013 to ASF-1016; https://appliedstemcell.com/mef-feeder-cells/ |
| Entry history | |
| Entry creation | 10-Apr-2025 |
| Last entry update | 10-Apr-2025 |
| Version number | 1 |