Cellosaurus DAc8-p53-1 (CVCL_E8CN)
| Cell line name | DAc8-p53-1 |
|---|---|
| Accession | CVCL_E8CN |
| Resource Identification Initiative | To cite this cell line use: DAc8-p53-1 (RRID:CVCL_E8CN) |
| Comments | Characteristics: Using homologous recombination a CAG-GFP-IRES-PuroR construct replaced exons 2 to 5 of Tp53 (PubMed=20703227). Knockout cell: Method=Homologous recombination; RGD; 3889; Tp53. Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Homologous recombination; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Rattus norvegicus (Rat)
(NCBI Taxonomy: 10116)
Breed/subspecies: Dark Agouti; RS=RS_0000014. |
| Hierarchy | Parent: CVCL_E220 (DAc8) |
| Sex of cell | Male |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=20703227; DOI=10.1038/nature09368; PMCID=PMC2937076 |
| Entry history | |
| Entry creation | 10-Apr-2025 |
| Last entry update | 14-Aug-2025 |
| Version number | 2 |