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Cellosaurus HEK293 ATG2A/2B DKO GFP-ATG2A(1-345) (CVCL_E8CI)

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Cell line name HEK293 ATG2A/2B DKO GFP-ATG2A(1-345)
Synonyms HEK293 ATG2A/2B DKO GFP-mini-ATG2A
Accession CVCL_E8CI
Resource Identification Initiative To cite this cell line use: HEK293 ATG2A/2B DKO GFP-ATG2A(1-345) (RRID:CVCL_E8CI)
Comments Characteristics: Transduced with a eGFP-mini-ATG2A (with only residues 1 to 345) construct (PubMed=30952800).
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:29028; ATG2A.
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:20187; ATG2B.
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Genetic integration: Method=Transduction; Gene= HGNC; HGNC:29028; ATG2A (Note=With p.Pro346_Asp1938del).
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_E8CG (HEK293 ATG2A/ATG2B DKO)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=30952800; DOI=10.1083/jcb.201811139; PMCID=PMC6548141
Valverde D.P., Yu S.-L., Boggavarapu V., Kumar N., Lees J.A., Walz T., Reinisch K.M., Melia T.J.
ATG2 transports lipids to promote autophagosome biogenesis.
J. Cell Biol. 218:1787-1798(2019)

Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1