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Cellosaurus iBSC1 (CVCL_E7ZK)

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Cell line name iBSC1
Synonyms iBSC; immortalized Bovine Satellite Cells 1
Accession CVCL_E7ZK
Resource Identification Initiative To cite this cell line use: iBSC1 (RRID:CVCL_E7ZK)
Comments Characteristics: Created by sleeping Beauty transposon-mediated integration of a PuroR-TERT-T2A-CDK4 construct (PubMed=37146268).
Doubling time: 17.4 hours (Note=At early passages), 15.1 hours (Note=At late passages) (PubMed=37146268).
Genetic integration: Method=Sleeping Beauty transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Genetic integration: Method=Sleeping Beauty transposition; Gene=VGNC; VGNC:111241; Bovine CDK4.
Genetic integration: Method=Sleeping Beauty transposition; Gene=VGNC; VGNC:35751; Bovine TERT.
Derived from site: In situ; Muscle, semitendinosus; UBERON=UBERON_0001375.
Cell type: Muscle satellite cell; CL=CL_0000594.
Species of origin Bos taurus (Bovine) (NCBI Taxonomy: 9913)
Breed/subspecies: Simmental; VBO=VBO_0000380.
Sex of cell Female
Age at sampling 5W
Category Telomerase immortalized cell line
Publications

PubMed=37146268; DOI=10.1021/acssynbio.3c00216
Andrew J. Stout, Miles J. Arnett, Kristin M. Chai, Tina Guo, Li-Shu Liao, Addison B. Mirliani, Miriam L. Rittenberg, Michelle Shub, Eugene C. White, John Se Kit Yuen Jr., Xiao-Li Zhang, David L. Kaplan;
Immortalized bovine satellite cells for cultured meat applications.
ACS Synth. Biol. 12:1567-1573(2023)

Cross-references
Cell line collections (Providers) Kerafast; ETU009
Entry history
Entry creation10-Apr-2025
Last entry update14-Aug-2025
Version number2