Cellosaurus cell line MCF-7/Luc-Akt-PH (CVCL

Cellosaurus MCF-7/Luc-Akt-PH (CVCL_E7HW)

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Cell line name

MCF-7/Luc-Akt-PH

Synonyms

MCF-7 Luc-Akt-PH

Accession

CVCL_E7HW

Resource Identification Initiative

To cite this cell line use: MCF-7/Luc-Akt-PH (RRID:CVCL_E7HW)

Comments

Population: Caucasian.
Characteristics: Transfected with a Luc-Akt-PH construct where the PH domain of mouse Akt1 is fused to Renilla luciferase (PubMed=24647478).
Genetic integration: Method=Transfection; Gene=MGI; MGI:87986; Akt1 (Note=With p.Lys111_Ala480del = PH domain).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P27652; Renilla reniformis renilla-type luciferase.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.

Sequence variations

Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; HGNC:4172; GATA3; Simple; p.Asp336Glyfs*17 (c.1006dupG); Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).

Disease

Invasive breast carcinoma of no special type (NCIt: C4194)

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Parent: CVCL_0031 (MCF-7)
Children:

CVCL_E7HX (MCF-7/B2)

Sex of cell

Female

Age at sampling

69Y