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Cellosaurus U2OS-CRISPR-TPR-SNAPf no.2-F01 (CVCL_E6B3)

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Cell line name U2OS-CRISPR-TPR-SNAPf no.2-F01
Synonyms U2OS-CRISPR-TPR-SNAPf #2-F01; U2OS CRISPR TPR-SNAPf #2-F01
Accession CVCL_E6B3
Resource Identification Initiative To cite this cell line use: U2OS-CRISPR-TPR-SNAPf no.2-F01 (RRID:CVCL_E6B3)
Comments Population: Caucasian.
Characteristics: Using CRISPR/Cas9D10A TPR has been endogenously tagged in both alleles at the C-terminus with a SNAP-f tag (PubMed=34191541).
Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:7059; MGMT (Note=With p.Gly183_Asn207del and 20 mutations = SNAP-f-tag).
Derived from site: In situ; Bone, tibia; UBERON=UBERON_0000979.
Sequence variations
  • Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Arg458Ter (c.1372C>T); ClinVar=VCV002445214; Zygosity=Heterozygous (from parent cell line).
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0042 (U2OS)
Sex of cell Female
Age at sampling 15Y
Category Cancer cell line
Publications

PubMed=34191541; DOI=10.1091/mbc.E20-11-0728; PMCID=PMC8351745
Vilma Jimenez Sabinina, M. Julius Hossain, Jean-Karim Heriche, Philipp Hoess, Bianca Nijmeijer-Winter, Shyamal Mosalaganti, Moritz Kueblbeck, Andrea Callegari, Anna Szymborska, Martin Beck, Jonas Ries, Jan Ellenberg;
Three-dimensional superresolution fluorescence microscopy maps the variable molecular architecture of the nuclear pore complex.
Mol. Biol. Cell 32:1523-1533(2021)

Cross-references
Cell line collections (Providers) CLS; 300667
Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1