Cellosaurus (CVCL_E5T5)
| Comments | Characteristics: Transfected with a pG-flox-CAGrtTA3G-IRES-Hyg-pA construct that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16. Genetic integration: Method=PiggyBac transposition; Gene=HGNC; HGNC:6347; KLF2. Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ). Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR). Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transfection; Gene=UniProtKB; P04486; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del). Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo. Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
|---|---|
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_ZJ91 (SEES3-1V) |
| Sex of cell | Male |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Entry history | |
| Entry creation | 02-Jul-2020 |
| Last entry update | 31-Mar-2026 |
| Version number | 12 |