Cellosaurus logo
expasy logo

Cellosaurus 1D3 [Mouse hybridoma against bovine CYCS] (CVCL_E5SJ)

[Text version]
Cell line name 1D3 [Mouse hybridoma against bovine CYCS]
Accession CVCL_E5SJ
Resource Identification Initiative To cite this cell line use: 1D3 [Mouse hybridoma against bovine CYCS] (RRID:CVCL_E5SJ)
Comments Monoclonal antibody isotype: IgG1, kappa.
Monoclonal antibody target: UniProtKB; P62894; Bovine CYCS (Note=Recognizes a conformational change that happens early in apoptosis and in necrosis. Also reacts with mouse and rat).
Anecdotal: Because of the loss of this hybridoma, a new recombinant mAB (R1D3) was created de novo using the sequence of remaining stocks of the mAB 1D3 (PubMed=36378644).
Miscellaneous: This hybridoma was lost in a defective liquid nitrogen tank (PubMed=36378644).
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Hierarchy Parent: CVCL_4032 (P3X63Ag8.653)
Category Hybridoma
Web pages Info; -; -; https://phys.org/news/2023-03-scientists-resurrect-dead-antibody-protein.html
Publications

PubMed=10090746; DOI=10.1021/bi9809268
Ronald Jemmerson, Jiang Liu, Danielle L. Hausauer, Kong-Peng Lam, Anna Mondino, R. David Nelson;
A conformational change in cytochrome c of apoptotic and necrotic cells is detected by monoclonal antibody binding and mimicked by association of the native antigen with synthetic phospholipid vesicles.
Biochemistry 38:3599-3609(1999)

PubMed=36378644; DOI=10.1073/pnas.2213432119; PMCID=PMC9704708
Florencia Tomasina, Jennyfer Martinez, Ari Zeida, Maria Laura Chiribao, Veronica Demicheli, Agustin Correa, Celia Quijano, Laura Castro, Robert H. Carnahan, Paige Newton Vinson, Matthew Goff ...Show all 21 authors... , Tracy Cooper, W. Hayes McDonald, Natalie Castellana, Luciana Hannibal, Paul Thomas Morse, Jun-Mei Wan, Maik Huttemann, Ronald Jemmerson, Lucia Piacenza, Rafael Radi; Show fewer authors
De novo sequencing and construction of a unique antibody for the recognition of alternative conformations of cytochrome c in cells.
Proc. Natl. Acad. Sci. U.S.A. 119:e2213432119.1-e2213432119.12(2022)

Entry history
Entry creation10-Apr-2025
Last entry update10-Apr-2025
Version number1