| Cell line name |
HCT 116 DNMT1(-/-) DNMT3B(-/-) |
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| Synonyms |
HCT116 DNMT1 (Delta exons3-5/Delta exons3-5) DNMT3B (-/-); HCT-116 double DNMT1-/- DNMT3b-/- KO |
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| Accession |
CVCL_E5EV |
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| Resource Identification Initiative |
To cite this cell line use: HCT 116 DNMT1(-/-) DNMT3B(-/-) (RRID:CVCL_E5EV) |
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| Comments |
Population: Caucasian.
Knockout cell: Method=Homologous recombination; HGNC; HGNC:2976; DNMT1.
Knockout cell: Method=Homologous recombination; HGNC; HGNC:2979; DNMT3B.
Derived from site: In situ; Colon; UBERON=UBERON_0001155. |
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| Sequence variations |
- Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
- Mutation; HGNC; HGNC:1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:3373; EP300; Simple; p.Asn1700Thrfs*9 (c.5099delA); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:9277; PPM1D; Simple; p.Leu450Ter (c.1349delT) (p.Leu450fs) (c.1344delT); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
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| Disease |
Colon carcinoma (NCIt: C4910) |
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| Species of origin |
Homo sapiens (Human)
(NCBI Taxonomy: 9606) |
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| Hierarchy |
Parent: CVCL_0291 (HCT 116) |
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| Sex of cell |
Male |
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| Age at sampling |
48Y |
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| Category |
Cancer cell line |
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| Publications | PubMed=11932749; DOI=10.1038/416552a
Ina Park Rhee, Kurtis E. Bachman, Ben Ho Park, Kam-Wing Jair, Ray-Whay Chiu Yen, Kornel E. Schuebel, Heng-Mi Cui, Andrew Paul Feinberg, Christoph Lengauer, Kenneth Wayne Kinzler, Stephen Bruce Baylin, Bert Vogelstein;
DNMT1 and DNMT3b cooperate to silence genes in human cancer cells.
Nature 416:552-556(2002) PubMed=12915469; DOI=10.1093/hmg/ddg226
Maria F. Paz, Susan Wei, Juan Cruz Cigudosa, Sandra Rodriguez-Perales, Miguel Angel Peinado, Tim Hui-Ming Huang, Manel Esteller;
Genetic unmasking of epigenetically silenced tumor suppressor genes in colon cancer cells deficient in DNA methyltransferases.
Hum. Mol. Genet. 12:2209-2219(2003) |
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| Cross-references |
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| Cell line collections (Providers) |
Horizon_Discovery; HD+R02-022
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| Entry history |
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| Entry creation | 10-Apr-2025 |
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| Last entry update | 10-Apr-2025 |
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| Version number | 1 |
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