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Cellosaurus EMC-G5 G5P.7A 3C9 (CVCL_E4IG)

[Text version]
Cell line name EMC-G5 G5P.7A 3C9
Synonyms G5P.7A subclone 3C9; 3C9
Accession CVCL_E4IG
Resource Identification Initiative To cite this cell line use: EMC-G5 G5P.7A 3C9 (RRID:CVCL_E4IG)
Comments Genetic integration: Method=Transfection; Gene=HGNC; HGNC:1069; BMP2.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:8568; FURIN (Note=Soluble version).
Genetic integration: Method=Transfection; Gene=MGI; MGI:94890; Dhfr.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Transformant: NCIt; C44386; Gamma radiation.
Omics: Proteomics; 2DE-MS.
Omics: Transcriptomics; Microarray.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Heterozygous (from parent cell line).
  • Mutation; UniProtKB; Q2MH30; Dhfr; Simple; p.Thr137Arg (c.410G>C); Zygosity=Heterozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_E4IF (EMC-G5)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=18240028; DOI=10.1007/s12033-008-9039-6
Padraig Doolan, Mark W. Melville, Patrick Gammell, Martin S. Sinacore, Paula Meleady, Kevin McCarthy, Linda Francullo, Mark W. Leonard, Timothy S. Charlebois, Martin Clynes;
Transcriptional profiling of gene expression changes in a PACE-transfected CHO DUKX cell line secreting high levels of rhBMP-2.
Mol. Biotechnol. 39:187-199(2008)

PubMed=18546152; DOI=10.1002/pmic.200700854
Paula Meleady, Michael Henry, Patrick Gammell, Padraig Doolan, Martin S. Sinacore, Mark W. Melville, Linda Francullo, Mark W. Leonard, Timothy S. Charlebois, Martin Clynes;
Proteomic profiling of CHO cells with enhanced rhBMP-2 productivity following co-expression of PACEsol.
Proteomics 8:2611-2624(2008)

Entry history
Entry creation19-Dec-2024
Last entry update14-Aug-2025
Version number3