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Cellosaurus CHO SSF3VN9Luc (CVCL_E4HZ)

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Cell line name CHO SSF3VN9Luc
Synonyms SSF3VN9Luc
Accession CVCL_E4HZ
Resource Identification Initiative To cite this cell line use: CHO SSF3VN9Luc (RRID:CVCL_E4HZ)
Comments Group: Serum/protein free medium cell line.
Characteristics: The expression of VTN is under transcriptional control of a DEX-inducible MMTV promoter (PubMed=10516577).
Genetic integration: Method=Transduction; Gene=MGI; MGI:94890; Dhfr.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:7978; NR3C1.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:12724; VTN.
Genetic integration: Method=Transfection; Gene=UniProtKB; P0A9M5; Escherichia coli gpt.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Transformant: NCIt; C44386; Gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Heterozygous (from parent cell line).
  • Mutation; UniProtKB; Q2MH30; Dhfr; Simple; p.Thr137Arg (c.410G>C); Zygosity=Heterozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_E4HX (CHO SSF3VN9)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=10516577; DOI=10.1002/(SICI)1097-0290(19991205)65:5<523::AID-BIT4>3.0.CO;2-6
Christine R. Gandor, Michael O. Zang-Gandor, Peter J. Flor, Marcello Marangi, Fred A.M. Asselbergs;
Conditionally adherent growth of serum-independent CHO cells for automated drug screening and biopharmaceutical production.
Biotechnol. Bioeng. 65:523-528(1999)

Entry history
Entry creation19-Dec-2024
Last entry update14-Aug-2025
Version number3