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Cellosaurus CHO E-10C10m (CVCL_E4HS)

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Cell line name CHO E-10C10m
Synonyms E-10C10m; E-10C101
Accession CVCL_E4HS
Resource Identification Initiative To cite this cell line use: CHO E-10C10m (RRID:CVCL_E4HS)
Comments Group: Patented cell line.
Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:5438; IFNG.
Genetic integration: Method=Transfection/transduction; Gene=MGI; MGI:94890; Dhfr.
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Transformant: NCIt; C44386; Gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Heterozygous (from parent cell line).
  • Mutation; UniProtKB; Q2MH30; Dhfr; Simple; p.Thr137Arg (c.410G>C); Zygosity=Heterozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_E4HN (CHO E-10C)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

Patent=EP0088540A2
Walter Charles Fiers;
DNA sequences, recombinant DNA molecules and processes for producing human immune interferon-like polypeptides.
Patent number EP0088540A2, 14-Sep-1983

PubMed=6308636; DOI=10.1073/pnas.80.15.4654; PMCID=PMC384102
Shaun J. Scahill, Rene Devos, Jose Van der Heyden, Walter Charles Fiers;
Expression and characterization of the product of a human immune interferon cDNA gene in Chinese hamster ovary cells.
Proc. Natl. Acad. Sci. U.S.A. 80:4654-4658(1983)

Patent=US5004689
Walter Charles Fiers, Bernard Allet;
DNA sequences, recombinant DNA molecules and processes for producing human gamma interferon-like polypeptides in high yields.
Patent number US5004689, 02-Apr-1991

Entry history
Entry creation19-Dec-2024
Last entry update19-Dec-2024
Version number1