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Cellosaurus CHO E-10C6 (CVCL_E4HQ)

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Cell line name CHO E-10C6
Synonyms E-10C6
Accession CVCL_E4HQ
Resource Identification Initiative To cite this cell line use: CHO E-10C6 (RRID:CVCL_E4HQ)
Comments Group: Patented cell line.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:5438; IFNG.
Genetic integration: Method=Transfection/transduction; Gene=MGI; MGI:94890; Dhfr.
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Transformant: NCIt; C44386; Gamma radiation.
Caution: It is uncertain whether the cell line used in PubMed=14705000 and PubMed=16003777 is CHO E-10C6 or another cell line derived from CHO EC-10B (Cellosaurus=CVCL_E4HM) or CHO EC-10C (Cellosaurus=CVCL_E4HN).
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Heterozygous (from parent cell line).
  • Mutation; UniProtKB; Q2MH30; Dhfr; Simple; p.Thr137Arg (c.410G>C); Zygosity=Heterozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_E4HN (CHO E-10C)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

Patent=EP0088540A2
Walter Charles Fiers;
DNA sequences, recombinant DNA molecules and processes for producing human immune interferon-like polypeptides.
Patent number EP0088540A2, 14-Sep-1983

PubMed=6308636; DOI=10.1073/pnas.80.15.4654; PMCID=PMC384102
Shaun J. Scahill, Rene Devos, Jose Van der Heyden, Walter Charles Fiers;
Expression and characterization of the product of a human immune interferon cDNA gene in Chinese hamster ovary cells.
Proc. Natl. Acad. Sci. U.S.A. 80:4654-4658(1983)

Patent=US5004689
Walter Charles Fiers, Bernard Allet;
DNA sequences, recombinant DNA molecules and processes for producing human gamma interferon-like polypeptides in high yields.
Patent number US5004689, 02-Apr-1991

PubMed=14705000; DOI=10.1002/bit.10861
Stephen R. Fox, Upasana A. Patel, Miranda Gek-Sim Yap, Daniel I-Chyau Wang;
Maximizing interferon-gamma production by Chinese hamster ovary cells through temperature shift optimization: experimental and modeling.
Biotechnol. Bioeng. 85:177-184(2004)

PubMed=16003777; DOI=10.1002/bit.20511
Katie Fraass Wlaschin, Peter Morin Nissom, Marcela de Leon Gatti, Peh-Fern Ong, Sanny Arleen, Kher-Shing Tan, Anette Rink, Breana Cham, Kathy Tin-Kam Wong, Miranda Gek-Sim Yap, Wei-Shou Hu;
EST sequencing for gene discovery in Chinese hamster ovary cells.
Biotechnol. Bioeng. 91:592-606(2005)

Entry history
Entry creation19-Dec-2024
Last entry update19-Dec-2024
Version number1