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Cellosaurus Jurkat-Lucia TCR-hPD-1 (CVCL_E3JE)

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Cell line name Jurkat-Lucia TCR-hPD-1
Accession CVCL_E3JE
Resource Identification Initiative To cite this cell line use: Jurkat-Lucia TCR-hPD-1 (RRID:CVCL_E3JE)
Comments Population: Caucasian.
Biotechnology: Used in the Jurkat-Raji PD-1/PD-L1 assay (Bio-IC) specifically designed for screening antibody-, Fc-fusion protein-, or small-molecule antagonists of the PD-1/PD-L1 immune checkpoint (IC) axis (Invivogen=rajkt-hpd1).
Characteristics: Express a specific TCR and PDCD1 (PD-1) along with the Lucia luciferase reporter gene under the control of an ISG54 minimal promoter fused to six NFAT response elements (InvivoGen=rajkt-hpd1).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:8760; PDCD1.
Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Genetic integration: Method=Transfection; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Mutation; HGNC; HGNC:959; BAX; Simple; p.Glu41Argfs*19 (c.121delG); ClinVar=VCV000009512; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:959; BAX; Simple; p.Glu41Glyfs*33 (c.121dupG); ClinVar=VCV000009511; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6079; INPP5D; Simple; p.Gln345Ter (c.1033C>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6079; INPP5D; Simple; c.1097+1065_1097+1112del47; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7325; MSH2; Simple; p.Arg711Ter (c.2131C>T); ClinVar=VCV000090903; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg196Ter (c.586C>T); ClinVar=VCV000043589; Zygosity=Heterozygous (from parent cell line).
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_KS47 (Jurkat-Lucia NFAT)
Sex of cell Male
Age at sampling 14Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) InvivoGen; rajkt-hpd1
Entry history
Entry creation19-Dec-2024
Last entry update10-Apr-2025
Version number2