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Cellosaurus CHO-K1 MUC1-IgG ST6GALNAC1 (CVCL_E3IY)

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Cell line name CHO-K1 MUC1-IgG ST6GALNAC1
Synonyms CHO MUC1 ST6GalNAc-I
Accession CVCL_E3IY
Resource Identification Initiative To cite this cell line use: CHO-K1 MUC1-IgG ST6GALNAC1 (RRID:CVCL_E3IY)
Comments Characteristics: The transfected MUC1 construct contains the first 16 tandem repeats of the extracellular domain of MUC1 followed by an enterokinase cleavage site (DDDDK) fusef to exon 1-3 of mouse IgG2a Fc (from parent cell line).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:7508; MUC1 (Note=With p.Ala381_Leu1255del).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:23614; ST6GALNAC1.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_E3IX (CHO-K1 MUC1-IgG)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=16319059; DOI=10.1074/jbc.M511826200
Robert Sewell, Malin Backstrom, Martin Dalziel, Stephen Gschmeissner, Hasse Karlsson, Thomas Noll, Jochem Gatgens, Henrik Clausen, Gunnar C. Hansson, Joy M. Burchell, Joyce Taylor-Papadimitriou;
The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.
J. Biol. Chem. 281:3586-3594(2006)

Entry history
Entry creation19-Dec-2024
Last entry update14-Aug-2025
Version number2