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Cellosaurus CHO TSHR-10,000 (CVCL_E3EM)

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Cell line name CHO TSHR-10,000
Synonyms TSHR-10,0000
Accession CVCL_E3EM
Resource Identification Initiative To cite this cell line use: CHO TSHR-10,000 (RRID:CVCL_E3EM)
Comments Characteristics: Adapted to growth in 10000 nM methotrexate (PubMed=8895321).
Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
Genetic integration: Method=Transfection; Gene=HGNC; HGNC:12373; TSHR (Note=With deleted untranslated regions).
Genetic integration: Method=Transfection; Gene=MGI; MGI:94890; Dhfr.
Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Homozygous (from parent cell line).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_E3EL (CHO TSHR-800)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Publications

PubMed=8895321; DOI=10.1210/endo.137.11.8895321
Gregorio D. Chazenbalk, Ayumu Kakinuma, Juan Carlos Jaume, Sandra M. McLachlan, Basil Rapoport;
Evidence for negative cooperativity among human thyrotropin receptors overexpressed in mammalian cells.
Endocrinology 137:4586-4591(1996)

Entry history
Entry creation19-Dec-2024
Last entry update19-Dec-2024
Version number1