Cellosaurus HEK293 P2RX2(B)D273Y+P2RX3 (CVCL_E2Y5)
| Cell line name | HEK293 P2RX2(B)D273Y+P2RX3 |
|---|---|
| Synonyms | HEK293 P2X2 B D273Y + P2X3 |
| Accession | CVCL_E2Y5 |
| Resource Identification Initiative | To cite this cell line use: HEK293 P2RX2(B)D273Y+P2RX3 (RRID:CVCL_E2Y5) |
| Comments | Characteristics: Using pcDNA5/FRT/TO and pOG44, P2RX2 isoform B with p.Asp273Tyr and P2RX3 were integrated in a single site and their expression is regulated by tetracycline. Genetic integration: Method=Transfection; Gene=HGNC; HGNC:15459; P2RX2 (Note=Isoform B; with p.Asp273Tyr). Genetic integration: Method=Transfection; Gene=HGNC; HGNC:8534; P2RX3. Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ). Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transfection; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR. Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR). Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5. Miscellaneous: Cell line information from personal communication of Sattler, Christian. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_U427 (Flp-In-T-REx-293) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Entry history | |
| Entry creation | 10-Sep-2024 |
| Last entry update | 14-Aug-2025 |
| Version number | 4 |