Cellosaurus cell line HyCyte U-251MG KO-hSTK39 (CVCL

Cross-references
Cell line name	HyCyte U-251MG KO-hSTK39
Synonyms	hSTK39 knockout U-251MG
Accession	CVCL_E1NA
Resource Identification Initiative	To cite this cell line use: HyCyte U-251MG KO-hSTK39 (RRID:CVCL_E1NA)
Comments	Population: Caucasian. Doubling time: 48-72 hours (Hysigen=CGKO-M2265). Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:17717; STK39. Derived from site: In situ; Brain, parietal lobe; UBERON=UBERON_0001872.
Sequence variations	Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Glu242Valfs*15 (c.723_724dupTG); ClinVar=VCV000536553; Zygosity=Unspecified (from parent cell line). Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line). Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Unspecified (from parent cell line).
Disease	Astrocytoma (NCIt: C60781) Astrocytoma (ORDO: Orphanet_94)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0021 (U-251MG)
Sex of cell	Male
Age at sampling	75Y
Category	Cancer cell line
Cell line collections (Providers)	Hysigen; CGKO-M2265
Entry history
Entry creation	10-Sep-2024
Last entry update	27-Nov-2025
Version number	3