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Cellosaurus MDA-MB-231 dCas9-VPR (CVCL_DX80)

[Text version]
Cell line name MDA-MB-231 dCas9-VPR
Accession CVCL_DX80
Resource Identification Initiative To cite this cell line use: MDA-MB-231 dCas9-VPR (RRID:CVCL_DX80)
Comments Group: Triple negative breast cancer (TNBC) cell line.
Population: Caucasian.
Characteristics: Transduced with a lentiviral vector EF1a-Bsd-T2A-dCas9-VPR-WPRE, where VPR is a tripartite activation system made of a fusion of VP64, p65(relA), and RTA (Horizon_Discovery=HD+dCas9-VPR-014).
Genetic integration: Method=Transduction; Gene=MGI; MGI:103290; Rela (Note=With p.Met1_Leu368del).
Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; Bacillus cereus Bsr.
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala = dCas9).
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Gene deletion; HGNC; HGNC:1788; CDKN2B; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Gly464Val (c.1391G>T); ClinVar=VCV000040364; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg280Lys (c.839G>A); ClinVar=VCV000376657; Zygosity=Homozygous (from parent cell line).
Disease Breast adenocarcinoma (NCIt: C5214)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0062 (MDA-MB-231)
Sex of cell Female
Age at sampling 51Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) Horizon_Discovery; HD+dCas9-VPR-014
Encyclopedic resources Wikidata; Q127382752
Entry history
Entry creation30-Jan-2024
Last entry update14-Aug-2025
Version number5