Cellosaurus cell line K-562 dCas9-VPR (CVCL

Cross-references
Cell line name	K-562 dCas9-VPR
Accession	CVCL_DX78
Resource Identification Initiative	To cite this cell line use: K-562 dCas9-VPR (RRID:CVCL_DX78)
Comments	Population: Caucasian. Characteristics: Transduced with a lentiviral vector EF1a-Bsd-T2A-dCas9-VPR-WPRE, where VPR is a tripartite activation system made of a fusion of VP64, p65(relA), and RTA (Horizon_Discovery=HD+dCas9-VPR-005). Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala = dCas9). Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). Transfected with: MGI:103290; Rela (with p.Met1_Leu368 del). Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations	Gene fusion; HGNC; 76; ABL1 + HGNC; 1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 14 fused to ABL1 exon 2 (b3a2 transcript) (from parent cell line). Mutation; HGNC; 11998; TP53; Simple; p.Gln136fs*13 (c.406_407insC); Zygosity=Homozygous (from parent cell line).
Disease	Blast phase chronic myelogenous leukemia, BCR-ABL1 positive (NCIt: C9110) Chronic myeloid leukemia (ORDO: Orphanet_521)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0004 (K-562)
Sex of cell	Female
Age at sampling	53Y
Category	Cancer cell line
Cell line collections (Providers)	Horizon_Discovery; HD+dCas9-VPR-005
Entry history
Entry creation	30-Jan-2024
Last entry update	02-May-2024
Version number	2