Cellosaurus A549 dCas9-VPR (CVCL_DX71)
| Cell line name | A549 dCas9-VPR |
|---|---|
| Accession | CVCL_DX71 |
| Resource Identification Initiative | To cite this cell line use: A549 dCas9-VPR (RRID:CVCL_DX71) |
| Comments | Population: Caucasian. Characteristics: Transduced with a lentiviral vector EF1a-Bsd-T2A-dCas9-VPR-WPRE, where VPR is a tripartite activation system made of a fusion of VP64, p65(relA), and RTA (Horizon_Discovery=HD+dCas9-VPR-001). Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (with p.Asp10Ala and p.His840Ala = dCas9). Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). Transfected with: MGI:103290; Rela (with p.Met1_Leu368 del). Derived from site: In situ; Lung; UBERON=UBERON_0002048. |
| Sequence variations |
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| Disease | Lung adenocarcinoma (NCIt: C3512) |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_0023 (A-549) |
| Sex of cell | Male |
| Age at sampling | 58Y |
| Category | Cancer cell line |
| Cross-references | |
| Cell line collections (Providers) | Horizon_Discovery; HD+dCas9-VPR-001 |
| Entry history | |
| Entry creation | 30-Jan-2024 |
| Last entry update | 30-Jan-2024 |
| Version number | 1 |