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Cellosaurus HA-CHO clone 4J (CVCL_DD05)

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Cell line name HA-CHO clone 4J
Synonyms 4J
Accession CVCL_DD05
Resource Identification Initiative To cite this cell line use: HA-CHO clone 4J (RRID:CVCL_DD05)
Comments Characteristics: Temperature defective (at 39 Celsius) expression of hemagglutinin (HA) on the cell surface (PubMed=2537314).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03451; Influenza A virus (strain A/Japan/305/1957 H2N2) hemagglutinin (HA).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_DD03 (HA-CHO)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications

PubMed=2537314; DOI=10.1083/jcb.108.2.339; PMCID=PMC2115425
Janet Hearing, Eric Hunter, Linda Rodgers, Mary-Jane Gething, Joseph Frank Sambrook;
Isolation of Chinese hamster ovary cell lines temperature conditional for the cell-surface expression of integral membrane glycoproteins.
J. Cell Biol. 108:339-353(1989)

PubMed=2267628; DOI=10.1007/BF01233094
Pamela J. Beck, Mary-Jane Gething, Joseph Frank Sambrook, Mark A. Lehrman;
Complementing mutant alleles define three loci involved in mannosylation of Man5-GlcNAc2-P-P-dolichol in Chinese hamster ovary cells.
Somat. Cell Mol. Genet. 16:539-548(1990)

Cross-references
Encyclopedic resources Wikidata; Q54872261
Entry history
Entry creation13-Jul-2016
Last entry update14-Aug-2025
Version number12