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Cellosaurus H4L1.1c4 (CVCL_DC27)

[Text version]
Cell line name H4L1.1c4
Synonyms H4IIE.Luc
Accession CVCL_DC27
Resource Identification Initiative To cite this cell line use: H4L1.1c4 (RRID:CVCL_DC27)
Comments Biotechnology: Cell line developed for CALUX (Chemically Activated LUciferase eXpression) bioassay. CALUX is used for rapid screening of samples for the presence of dioxin/TCDD-like compounds and AhR agonists/antagonists.
Characteristics: Acts through the activation of a luciferase reporter gene under the control of dioxin-response elements (DREs).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Disease Rat hepatocellular carcinoma (NCIt: C60416)
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: AxC; RS=RS_0000369.
Hierarchy Parent: CVCL_0284 (H4-II-E)
Sex of cell Male
Category Cancer cell line
Web pages Provider; XDSI; -; http://www.dioxins.com/products/
Publications

PubMed=8812260; DOI=10.1006/faat.1996.0152
Albertinka J. Murk, Juliette Legler, Michael S. Denison, John P. Giesy, Cees van de Guchte, Abraham Brouwer;
Chemical-activated luciferase gene expression (CALUX): a novel in vitro bioassay for Ah receptor active compounds in sediments and pore water.
Fundam. Appl. Toxicol. 33:149-160(1996)

PubMed=8812265; DOI=10.1006/faat.1996.0056
Patricia M. Garrison, Kathryn Tullis, Jac M.M.J.G. Aarts, Abraham Brouwer, John P. Giesy, Michael S. Denison;
Species-specific recombinant cell lines as bioassay systems for the detection of 2,3,7,8-tetrachlorodibenzo-p-dioxin-like chemicals.
Fundam. Appl. Toxicol. 30:194-203(1996)

Patent=US5854010
Michael S. Denison, Abraham Brouwer, George C. Clark;
Bioassay for detecting 2,3,7,8-tetrachlorodibenzo-para-dioxin and TCDD-like compounds and novel recombinant cell line useful therefor.
Patent number US5854010, 29-Dec-1998

PubMed=18969542; DOI=10.1016/j.talanta.2004.05.032
Michael S. Denison, Bin Zhao, David S. Baston, George C. Clark, Hiroshi Murata, Dalho Han;
Recombinant cell bioassay systems for the detection and relative quantitation of halogenated dioxins and related chemicals.
Talanta 63:1123-1133(2004)

PubMed=21394221; DOI=10.1007/s11426-010-0142-8; PMCID=PMC3049243
Bin Zhao, David S. Baston, Elaine Khan, Claudio Sorrentino, Michael S. Denison;
Enhancing the response of CALUX and CAFLUX cell bioassays for quantitative detection of dioxin-like compounds.
Sci. China Chem. 53:1010-1016(2010)

PubMed=26366531; DOI=10.1021/acs.est.5b02906; PMCID=PMC4772899
Jennifer C. Brennan, Guo-Chun He, Tomoaki Tsutsumi, Jing Zhao, Edward Wirth, Michael H. Fulton, Michael S. Denison;
Development of species-specific Ah receptor-responsive third generation CALUX cell lines with enhanced responsiveness and improved detection limits.
Environ. Sci. Technol. 49:11903-11912(2015)

Cross-references
Encyclopedic resources Wikidata; Q54872158
Entry history
Entry creation13-Jul-2016
Last entry update14-Aug-2025
Version number10