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Cellosaurus HEK293T_HTT null (CVCL_D7EP)

[Text version]
Cell line name HEK293T_HTT null
Accession CVCL_D7EP
Resource Identification Initiative To cite this cell line use: HEK293T_HTT null (RRID:CVCL_D7EP)
Comments Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:4851; HTT.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0063 (HEK293T)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
Publications

PubMed=33432339; DOI=10.1093/hmg/ddaa283; PMCID=PMC8248964
Roy Jung, Yejin Lee, Douglas Barker, Kevin Correia, Baehyun Shin, Jacob Loupe, Ryan Lewis Collins, Diane E. Lucente, Jayla Ruliera, Tammy Gillis, Jayalakshmi S. Mysore ...Show all 20 authors... , Lance Rodan, Jonathan Picker, Jong-Min Lee, David S. Howland, Ramee Lee, Seung Kwak, Marcy Elizabeth MacDonald, James Francis Gusella, Ihn Sik Seong; Show fewer authors
Mutations causing Lopes-Maciel-Rodan syndrome are huntingtin hypomorphs.
Hum. Mol. Genet. 30:135-148(2021)

PubMed=39257448; DOI=10.12688/f1000research.153670.1; PMCID=PMC11384196
Rebeka Fanti, Riham Ayoubi, Charles Alende, Maryam Fotouhi, Sara Gonzalez Bolivar, Renu Chandrasekaran, Kathleen Southern, Aled Morgan Edwards, Rachel Jane Harding, Carl Laflamme;
ABIF Consortium
NeuroSGC/YCharOS/EDDU Collaborative Group
A guide to selecting high-performing antibodies for huntingtin (UniProt ID: P42858) for use in western blot, immunoprecipitation, and immunofluorescence.
F1000Research 13:922.1-922.12(2024)

Entry history
Entry creation10-Sep-2024
Last entry update14-Aug-2025
Version number4