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Cellosaurus BJ SiPS-D TH-TdTomato DJ1 HET KO (CVCL_D5YD)

[Text version]
Cell line name BJ SiPS-D TH-TdTomato DJ1 HET KO
Accession CVCL_D5YD
Resource Identification Initiative To cite this cell line use: BJ SiPS-D TH-TdTomato DJ1 HET KO (RRID:CVCL_D5YD)
Comments Population: Caucasian.
Characteristics: Fluorescent (tdTomato) reporter cell line for TH expression. Using CRISPR/Cas9 a P2A-tdTomato construct was introduced in the C-terminal end of TH (from parent cell line).
Knockout cell: Method=CRISPR/Cas9; HGNC; 16369; PARK7 (Note=1 of 2 alleles).
Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed).
Derived from site: In situ; Foreskin, skin; UBERON=UBERON_0001471.
Cell type: Fibroblast of foreskin; CL=CL_1001608.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C8FN (BJ SiPS-D TH-TdTomato)
Sex of cell Male
Age at sampling <1M
Category Induced pluripotent stem cell
Publications

PubMed=38200091; DOI=10.1038/s41467-024-44732-2
Parfitt G.M., Coccia E., Goldman C., Whitney K., Reyes R., Sarrafha L., Nam K.H., Sohail S., Jones D.R., Crary J.F., Ordureau A., Blanchard J., Ahfeldt T.
Disruption of lysosomal proteolysis in astrocytes facilitates midbrain organoid proteostasis failure in an early-onset Parkinson's disease model.
Nat. Commun. 15:447.1-447.17(2024)

Entry history
Entry creation02-May-2024
Last entry update02-May-2024
Version number1