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Cellosaurus S12-27IIb (CVCL_D5XT)

[Text version]
Cell line name S12-27IIb
Accession CVCL_D5XT
Resource Identification Initiative To cite this cell line use: S12-27IIb (RRID:CVCL_D5XT)
Comments Group: Insect cell line.
Characteristics: Established from embryos carrying Act5C-GAL4 and UAS-Ras[V12].
Knockout cell: Method=Mutagenesis; FlyBase_Gene; FBgn0020887; Su(z)12 (Note=p.Gln219Ter mutation).
Genetic integration: Method=Transfection/transduction; Gene=FlyBase_Gene; FBgn0003205; Ras85D (Note=With p.Gly12Val).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04386; Yeast GAL4.
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Breed/subspecies: Su(z)12(4).
Sex of cell Sex unspecified
Age at sampling Embryonic stage
Category Spontaneously immortalized cell line
Publications

PubMed=27557709; DOI=10.1093/nar/gkw701; PMCID=PMC5137424
Tatyana G. Kahn, Eshagh Dorafshan Esfahani, Dorothea Schultheis, Aman Zare, Per Stenberg, Ingolf Reim, Vincenzo Pirrotta, Yuri B. Schwartz;
Interdependence of PRC1 and PRC2 for recruitment to Polycomb response elements.
Nucleic Acids Res. 44:10132-10149(2016)

Cross-references
Cell line collections (Providers) DGRC; 339
Cell line databases/resources FlyBase_Cell_line; FBtc0000339
Encyclopedic resources Wikidata; Q127384141
Entry history
Entry creation02-May-2024
Last entry update10-Sep-2024
Version number2