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Cellosaurus TBY2-31/ST (CVCL_D5GQ)

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Cell line name TBY2-31/ST
Accession CVCL_D5GQ
Resource Identification Initiative To cite this cell line use: TBY2-31/ST (RRID:CVCL_D5GQ)
Comments Group: Plant cell line.
Characteristics: Used to visualize the cis-Golgi network and trans-Golgi network thanks to the integration of two fusion constructs GFP-AtSYP31 and ST52-mRFP (PubMed=22740633; PubMed=28839076).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; 1XF1B; GFP (Note=avGFP; original A.victoria green fluorescent protein).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; 5YCFA; mRFP1 (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Genetic integration: Method=Transfection/transduction; Gene=RGD; 3676; St6gal1 (Note=With p.Met53_Cys403del = ST52 Cyto+TM).
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q9FFK1; Arabidopsis thaliana SYP31.
Derived from site: In situ; Seedling; PO=PO_0008037.
Species of origin Nicotiana tabacum (Common tobacco) (NCBI Taxonomy: 4097)
Breed/subspecies: Cultivar Bright Yellow 2.
Hierarchy Parent: CVCL_D5GT (TBY2-R31)
Category Spontaneously immortalized cell line
Publications

PubMed=22740633; DOI=10.1091/mbc.E12-01-0034; PMCID=PMC3418314
Yoko Ito, Tomohiro Uemura, Keiko Shoda, Masaru Fujimoto, Takashi Ueda, Akihiko Nakano;
Cis-Golgi proteins accumulate near the ER exit sites and act as the scaffold for Golgi regeneration after brefeldin A treatment in tobacco BY-2 cells.
Mol. Biol. Cell 23:3203-3214(2012)

PubMed=28839076; DOI=10.1242/jcs.203893
Yoko Ito, Tomohiro Uemura, Akihiko Nakano;
The Golgi entry core compartment functions as a COPII-independent scaffold for ER-to-Golgi transport in plant cells.
J. Cell Sci. 131:jcs203893.1-jcs203893.9(2018)

Cross-references
Cell line collections (Providers) RIKEN_BRC_EPD; rpc00091
Encyclopedic resources Wikidata; Q127384372
Entry history
Entry creation02-May-2024
Last entry update10-Apr-2025
Version number3