Cellosaurus HEK293T TetOff-SNCA-A53T mNeonGreen-3K-1-10-IRES-mKate2 (CVCL_D4BZ)
| Cell line name | HEK293T TetOff-SNCA-A53T mNeonGreen-3K-1-10-IRES-mKate2 |
|---|---|
| Synonyms | Syn10 split mNeonGreen |
| Accession | CVCL_D4BZ |
| Resource Identification Initiative | To cite this cell line use: HEK293T TetOff-SNCA-A53T mNeonGreen-3K-1-10-IRES-mKate2 (RRID:CVCL_D4BZ) |
| Comments | Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line). Characteristics: Additional expression of a plasmid encoding a bicistronic IRES construct of a split mNeonGreen (3K variant, B-strands 1-10) as well as an mKate2 marker (Direct_author_submission). Transfected with: HGNC; 11138; SNCA (with p.Ala53Thr). Transfected with: UniProtKB; P04483; Escherichia coli Tn10 tetR. Transfected with: UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (with p.Met1_Arg360del). Transfected with: mKate2, a bright red fluorescent protein derived from eqFP578. Transfected with: UniProtKB; A0A1S4NYF2; mNeonGreen (derivative of Branchiostoma lanceolatum blFP-Y3). Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd). Transfected with: UniProtKB; P13249; S.alboniger pac (PuroR). Transfected with: UniProtKB; P00552; Transposon Tn5 neo. Transformant: NCBI_TaxID; 28285; Adenovirus 5. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=tsA]. Miscellaneous: Cell line information from personal communication of Yuste-Checa P. Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_D4BW (HEK293T TetOff-SNCA-A53T) |
| Sex of cell | Female |
| Age at sampling | Fetus |
| Category | Transformed cell line |
| Entry history | |
| Entry creation | 30-Jan-2024 |
| Last entry update | 30-Jan-2024 |
| Version number | 1 |