Cellosaurus H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- Keima-REEP5 (CVCL_D1IS)
Cell line name | H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- Keima-REEP5 |
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Synonyms | H9 ES AAVS1-NGN2 FAM134C/A-/-; PiggyBac-Keima-REEP5 |
Accession | CVCL_D1IS |
Resource Identification Initiative | To cite this cell line use: H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/- Keima-REEP5 (RRID:CVCL_D1IS) |
Comments | Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (from parent cell line). Characteristics: Transduced with a PiggyBac transposon vector pAC150-PBLHL-4xHS-EF1a-Keima-REEP5 that localizes specifically to endoplasmic reticulum tubules (PubMed=37425907). Knockout cell: Method=CRISPR/Cas9; HGNC; 28450; RETREG2. Knockout cell: Method=CRISPR/Cas9; HGNC; 27258; RETREG3. Transfected with: HGNC; 13805; NEUROG2. Transfected with: HGNC; 30077; REEP5. Transfected with: UniProtKB; Q1JV70; Montipora sp. 20 fluorescent protein mKeima. Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_D1IB (H9 AAVS1-TRE3G-NGN2 RETREG3-/- RETREG2-/-) |
Sex of cell | Female |
Age at sampling | Blastocyst stage |
Category | Embryonic stem cell |
Publications | PubMed=37425907; DOI=10.1101/2023.06.26.546565 |
Entry history | |
Entry creation | 30-Jan-2024 |
Last entry update | 30-Jan-2024 |
Version number | 1 |