Cellosaurus H9 AAVS1-TRE3G-NGN2 RETREG1-/- Keima-REEP5 (CVCL_D1IN)
| Cell line name | H9 AAVS1-TRE3G-NGN2 RETREG1-/- Keima-REEP5 |
|---|---|
| Synonyms | H9 ES AAVS1-NGN2 FAM134B-/-; PiggyBac-Keima-REEP5 |
| Accession | CVCL_D1IN |
| Resource Identification Initiative | To cite this cell line use: H9 AAVS1-TRE3G-NGN2 RETREG1-/- Keima-REEP5 (RRID:CVCL_D1IN) |
| Comments | Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (from parent cell line). Characteristics: Transduced with a PiggyBac transposon vector pAC150-PBLHL-4xHS-EF1a-Keima-REEP5 that localizes specifically to endoplasmic reticulum tubules (PubMed=37425907). Knockout cell: Method=CRISPR/Cas9; HGNC; 25964; RETREG1. Transfected with: HGNC; 13805; NEUROG2. Transfected with: HGNC; 30077; REEP5. Transfected with: UniProtKB; Q1JV70; Montipora sp. 20 fluorescent protein mKeima. Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358. Cell type: Embryonic stem cell; CL=CL_0002322. |
| Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
| Hierarchy | Parent: CVCL_D1IF (H9 AAVS1-TRE3G-NGN2 RETREG1-/-) |
| Sex of cell | Female |
| Age at sampling | Blastocyst stage |
| Category | Embryonic stem cell |
| Publications | PubMed=37425907; DOI=10.1101/2023.06.26.546565 |
| Entry history | |
| Entry creation | 30-Jan-2024 |
| Last entry update | 30-Jan-2024 |
| Version number | 1 |