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Cellosaurus H9 AAVS1-TRE3G-NGN2 CCPG1-/- Keima-REEP5 (CVCL_D1IL)

[Text version]
Cell line name H9 AAVS1-TRE3G-NGN2 CCPG1-/- Keima-REEP5
Synonyms H9 ES AAVS1-NGN2 CCPG1-/-; PiggyBac-Keima-REEP5
Accession CVCL_D1IL
Resource Identification Initiative To cite this cell line use: H9 AAVS1-TRE3G-NGN2 CCPG1-/- Keima-REEP5 (RRID:CVCL_D1IL)
Comments Characteristics: Using CRISPR/Cas9 a TRE3G promoter driven NEUROG2 construct was introduced in the AAVS1 safe harbor locus (from parent cell line).
Characteristics: Transduced with a PiggyBac transposon vector pAC150-PBLHL-4xHS-EF1a-Keima-REEP5 that localizes specifically to endoplasmic reticulum tubules (PubMed=37425907).
Knockout cell: Method=CRISPR/Cas9; HGNC; 24227; CCPG1.
Transfected with: HGNC; 13805; NEUROG2.
Transfected with: HGNC; 30077; REEP5.
Transfected with: UniProtKB; Q1JV70; Montipora sp. 20 fluorescent protein mKeima.
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_D1IJ (H9 AAVS1-TRE3G-NGN2 CCPG1-/-)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell

PubMed=37425907; DOI=10.1101/2023.06.26.546565
Hoyer M.J., Smith I.R., Paoli J.C., Jiang Y.-Z., Paulo J.A., Harper J.W.
Combinatorial selective ER-phagy remodels the ER during neurogenesis.
bioRxiv 2023:06.26.546565-06.26.546565(2023)

Entry history
Entry creation30-Jan-2024
Last entry update30-Jan-2024
Version number1