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Cellosaurus MKL-2 (CVCL_D027)

[Text version]
Cell line name MKL-2
Synonyms Mkl-2; MKL2
Accession CVCL_D027
Resource Identification Initiative To cite this cell line use: MKL-2 (RRID:CVCL_D027)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Virology: Contains 1.7 copies of the genome of MCPyV per haploid cell (PubMed=23085629).
Doubling time: 5 days (PubMed=20444890); 3 days (PubMed=30873613).
Transformant: NCBI_TaxID; 493803; Merkel cell polyomavirus (MCPyV).
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Proteomics; Subcellular; Exosome.
Omics: Variations; Array-based CGH.
Disease Merkel cell carcinoma (NCIt: C9231)
Cutaneous neuroendocrine carcinoma (ORDO: Orphanet_79140)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 72Y
Category Cancer cell line
STR profile Source(s): PubMed=30873613

Markers:
AmelogeninX,Y
CSF1PO10,12
D3S135817
D5S81812,14
D7S82010,13
D8S117911,13
D13S31712,13
D16S53910,12
D18S5114,17
D21S1128.2,31.2
FGA19,26
Penta D10,13
Penta E11,13
TH017,8
TPOX8,11
vWA16,17

Run an STR similarity search on this cell line
Publications

PubMed=1717086; DOI=10.1016/0169-6009(91)90088-H
Estralita M.E. Martin, Victor E. Gould, Anders Hoog, Steven T. Rosen, James A. Radosevich, Leonard J. Deftos;
Parathyroid hormone-related protein, chromogranin A, and calcitonin gene products in the neuroendocrine skin carcinoma cell lines MKL1 and MKL2.
Bone Miner. 14:113-120(1991)

PubMed=12209990; DOI=10.1002/ijc.10591
Mireille Van Gele, Joan Helen Leonard, Nadine Van Roy, Heidi Van Limbergen, Simon Van Belle, Veronique F. Cocquyt, Helen R. Salwen, Anne De Paepe, Frank Speleman;
Combined karyotyping, CGH and M-FISH analysis allows detailed characterization of unidentified chromosomal rearrangements in Merkel cell carcinoma.
Int. J. Cancer 101:137-145(2002)

PubMed=20444890; DOI=10.1128/JVI.02400-09; PMCID=PMC2898224
Roland Houben, Masahiro Shuda, Rita Weinkam, David Schrama, Hui-Chen Feng, Yuan Chang, Patrick S. Moore, Jurgen C. Becker;
Merkel cell polyomavirus-infected Merkel cell carcinoma cells require expression of viral T antigens.
J. Virol. 84:7064-7072(2010)

PubMed=23085629; DOI=10.1016/j.jviromet.2012.10.001; PMCID=PMC3606892
Anna Guastafierro, Hui-Chen Feng, Mamie Thant, John Munn Kirkwood, Yuan Chang, Patrick S. Moore, Masahiro Shuda;
Characterization of an early passage Merkel cell polyomavirus-positive Merkel cell carcinoma cell line, MS-1, and its growth in NOD scid gamma mice.
J. Virol. Methods 187:6-14(2013)

PubMed=23563200; DOI=10.1038/jid.2013.169
Roland Houben, Christina Dreher, Sabrina Angermeyer, Andreas Borst, Jochen Sven Utikal, Sebastian Haferkamp, Wiebke K. Peitsch, David Schrama, Sonja Hesbacher;
Mechanisms of p53 restriction in Merkel cell carcinoma cells are independent of the Merkel cell polyoma virus T antigens.
J. Invest. Dermatol. 133:2453-2460(2013)

PubMed=27121059; DOI=10.18632/oncotarget.8793; PMCID=PMC5078066
Sonja Hesbacher, Lisa Pfitzer, Katharina Wiedorfer, Sabrina Angermeyer, Andreas Borst, Sebastian Haferkamp, Claus-Jurgen Scholz, Marion Wobser, David Schrama, Roland Houben;
RB1 is the crucial target of the Merkel cell polyomavirus Large T antigen in Merkel cell carcinoma cells.
Oncotarget 7:32956-32968(2016)

PubMed=27402257; DOI=10.1002/pmic.201600223
Aelita Konstantinell, Jack-Ansgar Bruun, Randi Olsen, Augusta Aspar, Natasa Skalko-Basnet, Baldur Sveinbjornsson, Ugo Moens;
Secretomic analysis of extracellular vesicles originating from polyomavirus-negative and polyomavirus-positive Merkel cell carcinoma cell lines.
Proteomics 16:2587-2591(2016)

PubMed=29696010; DOI=10.3389/fmicb.2018.00713; PMCID=PMC5905237
Celestino Velasquez, Yutaka Amako, Alexis Harold, Tuna Toptan, Yuan Chang, Masahiro Shuda;
Characterization of a Merkel cell polyomavirus-positive Merkel cell carcinoma cell line CVG-1.
Front. Microbiol. 9:713.1-713.10(2018)

PubMed=30873613; DOI=10.1002/ijc.32280
David Schrama, Eva-Maria Sarosi, Christian Adam, Cathrin Ritter, Ulrike Kaemmerer, Eva Klopocki, Eva-Maria Konig, Jochen Sven Utikal, Jurgen C. Becker, Roland Houben;
Characterization of six Merkel cell polyomavirus-positive Merkel cell carcinoma cell lines: integration pattern suggest that large T antigen truncating events occur before or during integration.
Int. J. Cancer 145:1020-1032(2019)

PubMed=31889939; DOI=10.1186/s12014-019-9263-z; PMCID=PMC6921584
Ulana Kotowski, Boban M. Erovic, Julia Schnoll, Victoria Stanek, Stefan Janik, Martin Steurer, Goran Mitulovic;
Quantitative proteome analysis of Merkel cell carcinoma cell lines using SILAC.
Clin. Proteomics 16:42.1-42.13(2019)

Cross-references
Cell line databases/resources Cell_Model_Passport; SIDM01790
DepMap; ACH-001123
Chemistry resources GDSC; 1333015
PharmacoDB; MKL2_935_2019
Encyclopedic resources Wikidata; Q54905817
Polymorphism and mutation databases Cosmic; 1693073
Cosmic; 2273917
Proteomic databases PRIDE; PXD004198
PRIDE; PXD008181
Entry history
Entry creation22-Oct-2012
Last entry update14-Aug-2025
Version number19