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Cellosaurus WAe001-A-1X (CVCL_C9LD)

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Cell line name WAe001-A-1X
Synonyms H1 MYH11-NLuc-tdTomato
Accession CVCL_C9LD
Resource Identification Initiative To cite this cell line use: WAe001-A-1X (RRID:CVCL_C9LD)
Comments Characteristics: Using CRISPR/Cas9 a Nanoluc-T2A-tdTomato-neo construct was integrated at the N-terminus of MYH11 (PubMed=31080110).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=CRISPR/Cas9; Gene=Oplophorus gracilirostris NanoLuc (Note=19 kDa ATP-independent bright luminescent protein derived from O.gracilirostris luciferase).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_9771 (WA01)
Sex of cell Male
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=31080110; DOI=10.1016/j.stemcr.2019.04.013; PMCID=PMC6565755
Jue Zhang, Brian E. McIntosh, Bowen Wang, Matthew E. Brown, Mitchell D. Probasco, Sarah Webster, Bret Duffin, Ying Zhou, Lian-Wang Guo, William J. Burlingham, Craig Kent ...Show all 13 authors... , Michael Ferris, James Alexander Thomson; Show fewer authors
A human pluripotent stem cell-based screen for smooth muscle cell differentiation and maturation identifies inhibitors of intimal hyperplasia.
Stem Cell Reports 12:1269-1281(2019)

Cross-references
Cell line databases/resources hPSCreg; WAe001-A-1X
Encyclopedic resources Wikidata; Q123033809
Entry history
Entry creation05-Oct-2023
Last entry update14-Aug-2025
Version number5