Cellosaurus cell line RKO/Cas9-hyg (CVCL

Cellosaurus RKO/Cas9-hyg (CVCL_C9ET)

Cross-references
Cell line name	RKO/Cas9-hyg
Accession	CVCL_C9ET
Resource Identification Initiative	To cite this cell line use: RKO/Cas9-hyg (RRID:CVCL_C9ET)
Comments	Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL539). Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations	Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs5 (c.1310delA); dbSNP=rs764719749; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs35 (c.383delA); ClinVar=VCV000477546; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Leu452Pro (c.1355T>C); ClinVar=VCV000930524; Zygosity=Heterozygous (from parent cell line).
Disease	Colon carcinoma (NCIt: C4910)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0504 (RKO)
Sex of cell	Sex unspecified
Age at sampling	Age unspecified
Category	Cancer cell line
Cell line collections (Providers)	GeneCopoeia; SL539
Encyclopedic resources	Wikidata; Q123033358
Entry history
Entry creation	05-Oct-2023
Last entry update	10-Apr-2025
Version number	5