Cellosaurus cell line C3A/Cas9-hyg (CVCL

Cross-references
Cell line name	C3A/Cas9-hyg
Accession	CVCL_C9DH
Resource Identification Initiative	To cite this cell line use: C3A/Cas9-hyg (RRID:CVCL_C9DH)
Comments	Population: Caucasian. Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL543). Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP). Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations	Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
Disease	Hepatoblastoma (NCIt: C3728) Hepatoblastoma (ORDO: Orphanet_449)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_1098 (Hep-G2/C3A)
Sex of cell	Male
Age at sampling	15Y
Category	Cancer cell line
Cell line collections (Providers)	GeneCopoeia; SL543
Encyclopedic resources	Wikidata; Q123030840
Entry history
Entry creation	05-Oct-2023
Last entry update	10-Apr-2025
Version number	5