Cellosaurus cell line CAMA-1/Cas9-hyg (CVCL

Cross-references
Cell line name	CAMA-1/Cas9-hyg
Accession	CVCL_C9DC
Resource Identification Initiative	To cite this cell line use: CAMA-1/Cas9-hyg (RRID:CVCL_C9DC)
Comments	Population: Caucasian. Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL562). Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP). Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations	Mutation; HGNC; 1748; CDH1; Simple; c.1712-1G>A; Zygosity=Homozygous; Note=Splice acceptor mutation (from parent cell line). Mutation; HGNC; 9588; PTEN; Simple; p.Asp92His (c.274G>C); Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; 11998; TP53; Simple; p.Arg280Thr (c.839G>C); ClinVar=VCV000012368; Zygosity=Homozygous (from parent cell line).
Disease	Breast adenocarcinoma (NCIt: C5214)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_1115 (CAMA-1)
Sex of cell	Female
Age at sampling	51Y
Category	Cancer cell line
Cell line collections (Providers)	GeneCopoeia; SL562
Encyclopedic resources	Wikidata; Q123030852
Entry history
Entry creation	05-Oct-2023
Last entry update	30-Jan-2024
Version number	2