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Cellosaurus DU4475/Cas9-hyg (CVCL_C9CJ)

[Text version]
Cell line name DU4475/Cas9-hyg
Accession CVCL_C9CJ
Resource Identification Initiative To cite this cell line use: DU4475/Cas9-hyg (RRID:CVCL_C9CJ)
Comments Group: Triple negative breast cancer (TNBC) cell line.
Population: Caucasian.
Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL547).
Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Derived from site: Metastatic; Skin; UBERON=UBERON_0002097.
Sequence variations
  • Gene deletion; HGNC; 6844; MAP2K4; Zygosity=Homozygous (from parent cell line).
  • Gene deletion; HGNC; 9884; RB1; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 583; APC; Simple; p.Glu1577Ter (c.4729G>T); Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
Disease Breast carcinoma (NCIt: C4872)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1183 (DU4475)
Sex of cell Female
Age at sampling 70Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL547
Encyclopedic resources Wikidata; Q123031193
Entry history
Entry creation05-Oct-2023
Last entry update02-May-2024
Version number3