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Cellosaurus MDA-MB-468/Cas9-hyg (CVCL_C9CF)

[Text version]
Cell line name MDA-MB-468/Cas9-hyg
Accession CVCL_C9CF
Resource Identification Initiative To cite this cell line use: MDA-MB-468/Cas9-hyg (RRID:CVCL_C9CF)
Comments Group: Triple negative breast cancer (TNBC) cell line.
Population: African American.
Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL516).
Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; c.253+1G>T; ClinVar=VCV000189475; Zygosity=Homozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; HGNC:9884; RB1; Simple; c.265_2787del2523; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
Disease Breast adenocarcinoma (NCIt: C5214)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0419 (MDA-MB-468)
Sex of cell Female
Age at sampling 51Y
Category Cancer cell line
Cross-references
Cell line collections (Providers) GeneCopoeia; SL516
Encyclopedic resources Wikidata; Q123033030
Entry history
Entry creation05-Oct-2023
Last entry update10-Apr-2025
Version number5