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Cellosaurus A549-deltaSTAT1-SunTag ACE2 (CVCL_C9AV)

[Text version]
Cell line name A549-deltaSTAT1-SunTag ACE2
Accession CVCL_C9AV
Resource Identification Initiative To cite this cell line use: A549-deltaSTAT1-SunTag ACE2 (RRID:CVCL_C9AV)
Comments Group: SARS-CoV-2 research cell line.
Population: Caucasian.
Characteristics: Doxycycline induces the expression of pCW-TRE-scFv-SunTag. SunTag consists of 24 copies of a short peptide epitope from yeast GCN4 and is used to recruite multiple protein copies to a polypeptide scaffold.
Knockout cell: Method=CRISPR/Cas9; HGNC; 11362; STAT1.
Transfected with: HGNC; 13557; ACE2.
Transfected with: UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Omics: CRISPR phenotypic screen.
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C9AU (A549-deltaSTAT1-SunTag)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
Publications

PubMed=35421191; DOI=10.1371/journal.ppat.1010464
Danziger O., Patel R.S., DeGrace E.J., Rosen M.R., Rosenberg B.R.
Inducible CRISPR activation screen for interferon-stimulated genes identifies OAS1 as a SARS-CoV-2 restriction factor.
PLoS Pathog. 18:E1010464-E1010464(2022)

Cross-references
Encyclopedic resources Wikidata; Q123030362
Entry history
Entry creation05-Oct-2023
Last entry update30-Jan-2024
Version number2