Cellosaurus logo
expasy logo

Cellosaurus A549-deltaSTAT1-SunTag ACE2 (CVCL_C9AV)

[Text][XML][JSON]
Cell line name A549-deltaSTAT1-SunTag ACE2
Accession CVCL_C9AV
Resource Identification Initiative To cite this cell line use: A549-deltaSTAT1-SunTag ACE2 (RRID:CVCL_C9AV)
Comments Group: SARS-CoV-2 research cell line.
Population: Caucasian.
Characteristics: Doxycycline induces the expression of pCW-TRE-scFv-SunTag. SunTag consists of 24 copies of a short peptide epitope from yeast GCN4 and is used to recruite multiple protein copies to a polypeptide scaffold.
Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:11362; STAT1.
Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:13557; ACE2.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P33967; Bacillus cereus Bsr.
Omics: Phenotyping; CRISPR screening.
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_C9AU (A549-deltaSTAT1-SunTag)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
Publications

PubMed=35421191; DOI=10.1371/journal.ppat.1010464; PMCID=PMC9041830
Oded Danziger, Roosheel Sandeep Patel, Emma J. DeGrace, Mikaela R. Rosen, Brad R. Rosenberg;
Inducible CRISPR activation screen for interferon-stimulated genes identifies OAS1 as a SARS-CoV-2 restriction factor.
PLoS Pathog. 18:e1010464.1-e1010464.31(2022)

Cross-references
Encyclopedic resources Wikidata; Q123030362
Entry history
Entry creation05-Oct-2023
Last entry update14-Aug-2025
Version number6