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Cellosaurus CALM/AF10 Cas9 (CVCL_C8ZE)

[Text version]
Cell line name CALM/AF10 Cas9
Synonyms Line #171
Accession CVCL_C8ZE
Resource Identification Initiative To cite this cell line use: CALM/AF10 Cas9 (RRID:CVCL_C8ZE)
Comments Characteristics: The CALM/AF10 oncogene was transduced into bone marrow hematopoietic stem cells and then transferred cells to sub-lethally irradiated recipients. Primary acute myeloid leukemia cells were harvested 3-6 months later, serially transplanted three times and then cultured in the presence of cytokines (PubMed=29478914).
Genetic integration: Method=Transduction; Gene=UniProtKB; P33967; Bacillus cereus Bsr.
Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Omics: Phenotyping; CRISPR screening.
Disease Mouse leukemia (NCIt: C21604)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Sex of cell Male
Category Cancer cell line
Publications

PubMed=29478914; DOI=10.1016/j.ccell.2018.01.012; PMCID=PMC5849534
Takuji Yamauchi, Takeshi Masuda, Matthew C. Canver, Michael Seiler, Yuichiro Semba, Mohammad Shboul, Mohammed Al-Raqad, Manami Maeda, Vivien A.C. Schoonenberg, Mitchel A. Cole, Claudio Macias-Trevino ...Show all 22 authors... , Yuichi Ishikawa, Qiu-Ming Yao, Michitaka Nakano, Fumio Arai, Stuart Holland Orkin, Bruno Reversade, Silvia Buonamici, Luca Pinello, Koichi Akashi, Daniel Evan Bauer, Takahiro Maeda; Show fewer authors
Genome-wide CRISPR-Cas9 screen identifies leukemia-specific dependence on a pre-mRNA metabolic pathway regulated by DCPS.
Cancer Cell 33:386-400.e5(2018)

Cross-references
CRISP screens repositories BioGRID_ORCS_Cell_line; 815
Encyclopedic resources Wikidata; Q123030850
Entry history
Entry creation05-Oct-2023
Last entry update14-Aug-2025
Version number6