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Cellosaurus A549 PPM1H-BromoTag (CVCL_C8YV)

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Cell line name A549 PPM1H-BromoTag
Synonyms A549 PPM1H-BromoTag CRISPR/Cas9 knock-in
Accession CVCL_C8YV
Resource Identification Initiative To cite this cell line use: A549 PPM1H-BromoTag (RRID:CVCL_C8YV)
Comments Population: Caucasian.
Characteristics: Using CRISPR/Cas9 PPM1H was tagged on both alleles at the C-terminus with a BromoTag thus enabling the rapid and selective degradation of PPM1H (DOI=10.1101/2023.07.25.550405).
Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:13575; BRD4 (Note=With p.Met1_Lys332del, p.Pro461_Phe1362del and p.Leu387Ala = BromoTag).
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0023 (A-549)
Sex of cell Male
Age at sampling 58Y
Category Cancer cell line
Publications

DOI=10.1101/2023.07.25.550405
Toan K. Phung, Kerryn Berndsen, Tran Le Cong Huyen Bao Phan, Miratul Mohamid Khan Muqit, Dario Renato Alessi, Raja Sekhar Nirujogi;
CURTAIN -- a unique Web-based tool for exploration and sharing of MS-based proteomics data.
bioRxiv 2023:07.25.550405-07.25.550405(2023)

Cross-references
Encyclopedic resources Wikidata; Q123030359
Entry history
Entry creation05-Oct-2023
Last entry update19-Dec-2024
Version number5