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Cellosaurus ML-DmBG3-c2 attP 99F8 (CVCL_C7G4)

[Text version]
Cell line name ML-DmBG3-c2 attP 99F8
Synonyms ML-DmBG3-c2 attP 99F8 Clone 28
Accession CVCL_C7G4
Resource Identification Initiative To cite this cell line use: ML-DmBG3-c2 attP 99F8 (RRID:CVCL_C7G4)
Comments Group: Insect cell line.
Characteristics: Using CRISPR/Cas9 a Actin5C promoter-dsRed cassette flanked by attP sites was introduced in the 99F8 locus (PubMed=33963853).
Transfected with: tdTomato, a bright red fluorescent protein derived from drFP583 (DsRed).
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Hierarchy Parent: CVCL_Z728 (ML-DmBG3-c2)
Sex of cell Male
Age at sampling Third instar larval stage
Category Spontaneously immortalized cell line

PubMed=33963853; DOI=10.1093/g3journal/jkab161
Mariyappa D., Luhur A., Overton D., Zelhof A.C.
Generation of Drosophila attP containing cell lines using CRISPR-Cas9.
G3 (Bethesda) 11:jkab161.1-jkab161.8(2021)

Cell line collections (Providers) DGRC; 321
Cell line databases/resources FlyBase_Cell_line; FBtc0000321
Encyclopedic resources Wikidata; Q117704713
Entry history
Entry creation21-Mar-2023
Last entry update05-Oct-2023
Version number3